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LYK-1
LYK-1
規(guī)格:
貨期:
編號(hào):B162436
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) LYK-1
商品貨號(hào) B162436
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: S344NCR (B cell); BALB/c (myeloma)

Applications
Animals were immunized with a fusion protein corresponding to the products of CD44 variable exons V5 through V10.
The antibody is specific for a determinant on murine CD44 isoforms expressing variable exon V10.
Derivation
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a determinant on murine CD44 isoforms expressing variable exon V10.
Cellular Products
immunoglobulin; monoclonal antibody; against a determinant on murine CD44 isoforms expressing variable exon V10.
Comments
Animals were immunized with a fusion protein corresponding to the products of CD44 variable exons V5 through V10.
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
The antibody is specific for a determinant on murine CD44 isoforms expressing variable exon V10.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate and supplemented with: 0.05 mM 2-mercaptoethanol 1% Origen Hybridoma Cloning Factor 10% fetal bovine serum
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 1 X 10(5) and 8 X 10(5) viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1
Name of Depositor Y Yamashita, KS Hathcock
Deposited As rat (B cell); mouse (myeloma)
References

Yamashita Y, et al. Tissue-specific and growth-regulated expression of CD44 variable exon determinants in the mouse. Cell. Immunol. 176: 22-33, 1997. PubMed: 9070314

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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