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Human herpesvirus 5
Human herpesvirus 5
規(guī)格:
貨期:
編號(hào):B163020
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Human herpesvirus 5
商品貨號(hào) B163020
Classification Herpesviridae, Cytomegalovirus
Deposited As Cytomegalovirus
Agent Human herpesvirus 5
Strain Towne
Common Name Human cytomegalovirus
Applications
Quality control
Sexually Transmitted Disease Research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Shipping Information Infected viable cells + 7% DMSO
Storage Conditions Liquid nitrogen vapor phase
Comments
Add contents of thawed ampule to a 80-85% monolayer of HDF cells. If foci of CPE fail to spread by day 11 PI, split infected cells 1:2 and observe for CPE as monolayer reforms.
Attenuated for man, broad antigenicity. Stable for 6 months at -60°C in form of glycerinated cells. Virus primarily cell associated. A long (10-11 day) incubation is required for initial passage of frozen stocks.
Effect on Host
CPE, cells round, become refractile, eventually slough off the flask
Recommended Host
MRC-5 (ATCC® CCL-171™)
Growth Conditions
Temperature: 37°C
Recommendations For Infection: Trypsinize a 2-4 day old cell culture and seed at a density of 105 cells per cm2 (2.5X106 cells/T25 flask; 7.5X 105 cells per T75 flask) using the virus growth medium (VGM). To the freshly trypsinized cells inoculate using a MOI of 0.1. Immediately add additional VGM depending upon the size of the flask used (recommended medium volume for T25 is 5 to 7 mL and for T75 15 to 22.5 mL). Incubate for 24 hours at 37°C in a 5% CO2 atmosphere. Aspirate media and add fresh virus/cell growth medium to remove any traces of DMSO. Continue incubation.
Incubation: 2-7 days in a 5% CO2 air atmosphere in a stationary position until CPE is seen
Name of Depositor SA Plotkin
Special Collection NCRR Contract
Source Urine, man with congenital cytomegalic inclusion disease
References

Plotkin SA, et al. Candidate cytomegalovirus strain for human vaccination. Infect. Immun. 12: 521-527, 1975. PubMed: 170203

Quality Control of Microbiological Transport Systems; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M40-A.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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