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Vaccinia virus ts mutant
Vaccinia virus ts mutant
規(guī)格:
貨期:
編號:B163394
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Vaccinia virus ts mutant
商品貨號 B163394
Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus ts mutant
Strain IHD-W Dts38
Applications
Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses
The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D5, an ATPase required for DNA replication.
Biosafety Level 2 BSL 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
This conditional lethal mutant, originally designated plaque isolate no. 6389, was isolated from the IHD-W strain of Vaccinia by S. Dales. The IHD-W strain was derived from an IHD-J strain of Vaccinia.
Product Format frozen
Storage Conditions -70°C or colder
Comments
This conditional lethal mutant, originally designated plaque isolate no. 6389, was gnerated from the IHD-W strain of Vaccinia by S. Dales by mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. It was assigned to Dales EM category C, indicating that it induces rudimentary virus membranes with spicules in foci of viroplasm in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D5, an ATPase required for DNA replication. Shifting infection conditions from permissive to non-permissive temperature results in an abrupt cessation of DNA synthesis, described as a "fast stop" phenotype. Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses
Effect on Host
Cytopathic effects in culture include enlargement and rounding of cells, followed by cell death and sloughing
CPE: enlargement, plaques with rounding and eventual sloughing
Growth Medium ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
Recommended Host
Production Host: BSC-40 (ATCC CRL-2761)

Recommended Host: BSC-40 (ATCC CRL-2761)

Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Growth Conditions
1-3 days at 31 C with 55 CO2
Name of Depositor S Dales, R. Condit
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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