国产精品国产精品一区精品国产自在现偷99精品国产在热2019国产拍偷精品网国产精品视频全国免费观看,国产精品v欧美精品v日韩精品青青精品视频国产久久国产精品久久精品国产亚洲精品国产精品国产欧美精品一区二区三区,国产精品第一页国产亚洲精品国产福利国产精品自拍国产精品视频在线观看亚洲国产精品一区二区久久国产精品国产三级国产专不,国产精品视频大陆精大陆国产国语精品2019精品国产品对白在线285年香蕉精品国产高清自在自线隔壁老王国产在线精品在线观看精品国产福利片,国产三级精品三级在专区精品国产自在现偷国产精品一区二区三区国产日韩精品欧美一区喷水亚洲精品国产精品国自产国产在线精品一区二区不卡

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > 20C2 [4H12:20C2.G6.C9]
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
20C2 [4H12:20C2.G6.C9]
20C2 [4H12:20C2.G6.C9]
規(guī)格:
貨期:
編號(hào):B163639
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 20C2 [4H12:20C2.G6.C9]
商品貨號(hào) B163639
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The hybridoma cell line 20C2 [4H12:20C2.G6.C9} was established by Richard Chizzonite in 1991 and subcloned three times by limiting dilution.
The hybridoma produces an antibody (IgG1) that binds the p75 heterodimer of human IL-12.
The antibody may be used in neutralizing and receptor binding assays; it will immunoprecipitate IL-12.
The antibody can be used as a capture antibody in sandwich EIA to detect the p75 form of human IL-12.
Storage Conditions liquid nitrogen vapor phase
Derivation
The hybridoma cell line 20C2 [4H12:20C2.G6.C9} was established by Richard Chizzonite in 1991 and subcloned three times by limiting dilution.
The 20C2 cell line was formed by the fusion of NSO mouse myeloma cells with splenocytes from a Lewis rat inoculated with partially purified human IL-12 p75.
Genes Expressed
immunoglobulin; monoclonal antibody; against human interleukin 12 (IL-12) p75
Cellular Products
immunoglobulin; monoclonal antibody; against human interleukin 12 (IL-12) p75
Comments
The hybridoma cell line 20C2 [4H12:20C2.G6.C9} was established by Richard Chizzonite in 1991 and subcloned three times by limiting dilution.
The hybridoma produces an antibody (IgG1) that binds the p75 heterodimer of human IL-12.
The antibody may be used in neutralizing and receptor binding assays; it will immunoprecipitate IL-12.
The antibody can be used as a capture antibody in sandwich EIA to detect the p75 form of human IL-12.
The 20C2 antibody does not bind the p40 or p35 subunits of IL-12.
The 20C2 cell line was formed by the fusion of NSO mouse myeloma cells with splenocytes from a Lewis rat inoculated with partially purified human IL-12 p75.
Complete Growth Medium Iscove's Modified Dulbecco's Medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.1 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%.
Subculturing Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (as cell density increases)

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG1
Name of Depositor DH Presky
Deposited As rat (B cell); mouse (myeloma)
Year of Origin 1991
References

Chua AO, et al. Expression cloning of a human IL-12 receptor component--a new member of the cytokine receptor superfamily with strong homology to gp130. J. Immunol. 153: 128-136, 1994. PubMed: 7911493

Chizzonite R, et al. IL-12: monoclonal antibodies specific for the 40-kDa subunit block receptor binding and biologic activity on activated human lymphoblasts. J. Immunol. 147: 1548-1556, 1991. PubMed: 1715362

Chizzonite R, et al. IL-12 receptor. I. Characterization of the receptor on phytohemagglutinin-activated human lymphoblasts. J. Immunol. 148: 3117-3124, 1992. PubMed: 1578138

Desai BB, et al. IL-12 receptor. II. Distribution and regulation of receptor expression. J. Immunol. 148: 3125-3132, 1992. PubMed: 1578139

Gately MK, et alMeasurement of Human and Mouse Interleukin-12In: Gately MK, et alCurrent Protocols in ImmunologyNew York, N.Y.John Wiley and Sons1995.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
四川省| 连城县| 塔河县| 锡林浩特市| 武宁县| 德昌县| 陆丰市| 福鼎市| 铜鼓县| 仪陇县| 井研县| 中方县| 五寨县| 武清区| 彭山县| 丹棱县| 泰和县| 清镇市| 仪征市| 泊头市| 长春市| 武宁县| 罗源县| 龙海市| 桐城市| 清远市| 宾川县| 吴旗县| 霍邱县| 丹巴县| 且末县| 曲松县| 安徽省| 南宫市| 泰宁县| 江门市| 平度市| 买车| 南阳市| 建始县| 通海县|