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4F2C13
4F2C13
規(guī)格:
貨期:
編號:B163770
品牌:Mingzhoubio

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產(chǎn)品名稱 4F2C13
商品貨號 B163770
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Leukemia
Applications
This line is derived from the same fusion used to produce ATCC HB-2 and ATCC HB-21.
The antibody reacts with peripheral blood monocytes and to a lesser extent peripheral blood T cells.
The antibody fixes complement and binds to Protein A.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
This line is derived from the same fusion used to produce ATCC HB-2 and ATCC HB-21.
Genes Expressed
immunoglobulin; monoclonal antibody; against human monocyte
Cellular Products
immunoglobulin; monoclonal antibody; against human monocyte
Tumorigenic Yes
Effects
Yes, in pristane primed BALB/c mice
Comments
Animals were immunized with HSB-2 cells (ATCC CCL-120.1, an acute lymphocytic T leukemia cell line).
Spleen cells were fused with P3X63Ag8 myeloma cells.
This line is derived from the same fusion used to produce ATCC HB-2 and ATCC HB-21.
The antibody reacts with peripheral blood monocytes and to a lesser extent peripheral blood T cells.
The antibody fixes complement and binds to Protein A.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium.
Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Isotype IgG2a; kappa light chain
Name of Depositor A Fauci, BF Haynes
Deposited As mouse (B cell); mouse (myeloma)
References

Eisenbarth GS, et al. Production of monoclonal antibodies reacting with peripheral blood mononuclear cell surface differentiation antigens. J. Immunol. 124: 1237-1244, 1980. PubMed: 6153676

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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