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70Z/3 [70Z/3.12]
70Z/3 [70Z/3.12]
規(guī)格:
貨期:
編號:B163808
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 70Z/3 [70Z/3.12]
商品貨號 B163808
Organism Mus musculus, mouse
Cell Type pre-B lymphoblast; methylnitrosourea induce
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Storage Conditions liquid nitrogen vapor phase
Comments
The cells are negative for surface immunoglobulin and terminal deoxynucleotidyl transferase (sIg -, TdT -).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 0.05 mM 2-mercaptoethanol

Subculturing Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions

Temperature: 37°C
Atmosphere: 5% CO2

Name of Depositor PW Kincade
Deposited As Mus musculus
References

Ralph P. T and B lymphocytes. New York: Academic Press; 1979.

Baines P, et al. Characterization of malignant cell populations in MNU-induced leukaemia of mice. Leuk. Res. 3: 23-28, 1979. PubMed: 316844

Paige CJ, et al. Murine B cell leukemia line with inducible surface immunoglobulin expression. J. Immunol. 121: 641-647, 1978. PubMed: 308074

Paige CJ, et al. Independent control of immunoglobulin heavy and light chain expression in a murine pre-B-cell line. Nature 292: 631-633, 1981. PubMed: 6789213

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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