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807.33
807.33
規(guī)格:
貨期:
編號(hào):B163837
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 807.33
商品貨號(hào) B163837
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The hybridoma cell line 807.33 secretes a mouse monoclonal antibody (IgG2a) reactive with the G1 protein of La Crosse Virus.
This antibody as well as 807.31 (ATCC CRL-2282) and 807.15 (ATCC CRL-2287) is useful for identifying infection by La Crosse virus and other California serogroup Bunyaviruses.
The 807.33 antibody has been used in ELISA, Western Blots, immunoprecipitation, immunofluorescence and neutralization assays.
The line was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with La Crosse Virus.
Storage Conditions liquid nitrogen vapor phase
Derivation
The line was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with La Crosse Virus.
Genes Expressed
immunoglobulin; monoclonal antibody; against the G1 protein of La Crosse Virus.
Cellular Products
immunoglobulin; monoclonal antibody; against the G1 protein of La Crosse Virus.
Comments
The hybridoma cell line 807.33 secretes a mouse monoclonal antibody (IgG2a) reactive with the G1 protein of La Crosse Virus.
This antibody as well as 807.31 (ATCC CRL-2282) and 807.15 (ATCC CRL-2287) is useful for identifying infection by La Crosse virus and other California serogroup Bunyaviruses.
The 807.33 antibody has been used in ELISA, Western Blots, immunoprecipitation, immunofluorescence and neutralization assays.
The antibody does not react with the G2 or L proteins of La Crosse virus.
The line was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with La Crosse Virus.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG2a
Name of Depositor F Gonzalez-Scarano
References

Gonzalez-Scarano F, et al. Characterization of monoclonal antibodies against the G1 and N proteins of LaCrosse and Tahyna, two California serogroup bunyaviruses. Virology 120: 42-53, 1982. PubMed: 7101727

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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