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The tissue donor suffered from Marfan syndrome.

female

The tissue donor suffered from Marfan syndrome.

There was no prior family history of Marfan syndrome.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 7 to 9 days.