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C3A [HepG2/C3A, derivative of Hep G2 (ATCC HB-8065)] (ATCC<sup>&reg;</sup> CRL-10741<sup>&trade;</sup>)
C3A [HepG2/C3A, derivative of Hep G2 (ATCC HB-8065)] (ATCC<sup>&reg;</sup> CRL-10741<sup>&trade;</sup>)
規(guī)格:
貨期:
編號(hào):B164087
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) C3A [HepG2/C3A, derivative of Hep G2 (ATCC HB-8065)] (ATCC® CRL-10741)
商品貨號(hào) B164087
Organism Homo sapiens, human
Tissue liver
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease hepatocellular carcinoma
Age 15 years adolescent
Gender male
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images ATCC CRL-10741 Cell Micrograph
Derivation
C3A is clonal derivative of Hep G2 that was selected for strong contact inhibition of growth, high albumin production, high production of alpha fetoprotein (AFP) and ability to grow in glucose deficient medium.
Clinical Data
15 years adolescent
Caucasian
male
Genes Expressed
alpha-fetoprotein (alpha fetoprotein); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); transferrin; alpha1 antichymotrypsin; (alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen;,complement (C4); C3 activator; fibrinogen; alpha1 acid glycoprotein (alpha-1 acid glycoprotein); alpha2 HS glycoprotein (alpha-2-HS-glycoprotein); beta lipoprotein (beta-lipoprotein); retinol binding protein (retinol-binding protein)
Cellular Products
alpha-fetoprotein (alpha fetoprotein); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); transferrin; alpha1 antichymotrypsin; (alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen;
complement (C4); C3 activator; fibrinogen; alpha1 acid glycoprotein (alpha-1 acid glycoprotein); alpha2 HS glycoprotein (alpha-2-HS-glycoprotein); beta lipoprotein (beta-lipoprotein); retinol binding protein (retinol-binding protein)
Tumorigenic No
Effects
No, in immunosuppressed mice
Yes, in semisolid medium
Comments

As the cells become confluent, there is a marked reduction in AFP secretion and an increase in albumin secretion.

Gluconeogenesis activity is strongly oxgen dependent.

The cells have nitrogen metabolizing activity comparable to perfused rat livers.

There is no evidence of a Hepatitis B virus genome in this cell line.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
    Medium Renewal: Twice per week
    Cryopreservation
    Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Temperature: 37°C
    STR Profile
    Amelogenin: X,Y
    CSF1PO: 10,11
    D13S317: 9,13
    D16S539: 12,13
    D5S818: 11,13
    D7S820: 10
    THO1: 9
    TPOX: 8,9
    vWA: 17
    Name of Depositor Baylor College of Medicine
    Deposited As Homo sapiens
    U.S. Patent Number
    References

    Kelly JH. Permanent human hepatocyte cell line and its use in a liver assist device (LAD). US Patent 5,290,684 dated Mar 1 1994

    梅經(jīng)理 17280875617 1438578920
    胡經(jīng)理 13345964880 2438244627
    周經(jīng)理 17757487661 1296385441
    于經(jīng)理 18067160830 2088210172
    沈經(jīng)理 19548299266 2662369050
    李經(jīng)理 13626845108 972239479
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