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The tissue donor suffered from cystic fibrosis.

male

Homozygous for the Delta 508 mutation in the CFTR gene.

The tissue donor suffered from cystic fibrosis.

Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended

Medium Renewal: 3 times per week

Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.