| 產(chǎn)品名稱(chēng) |
CSMalphabeta6C [CSMab6C] |
| 商品貨號(hào) |
B164295 |
| Organism |
Mus musculus, mouse |
| Tissue |
mammary gland |
| Cell Type |
fibroblast |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Papilloma |
| Gender |
female |
| Strain |
RIII |
| Applications |
produces chorionic gonadotropin, beta polypeptide produces glycoprotein hormones, alpha polypeptide chorionic gonadotropin, alpha polypeptide, luteinizing hormone alpha |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
This line was derived from the C127I mouse fibroblast cell line by transformation with a bovine papilloma virus vector containing the alpha-hCG gene under control of the SV40 early promoter and beta-hCG under control of the metallothionine promoter. |
| Clinical Data |
female |
| HeLa Markers |
N |
| Genes Expressed |
glycoprotein hormones, alpha polypeptide(chorionic gonadotropin, alpha polypeptide, luteinizing hormone alpha) [CGA],chorionic gonadotropin, beta polypeptide, glycoprotein hormones, alpha polypeptide |
| Comments |
This line was derived from the C127I mouse fibroblast cell line by transformation with a bovine papilloma virus vector containing the alpha-hCG gene under control of the SV40 early promoter and beta-hCG under control of the metallothionine promoter. The cells produce very high levels of alpha and beta hCG. The line contains the plasmid pRF 398 alpha t2. The construct contains the entire sequence of beta hCG plus 30 bp 5' untranslated and 60 bp 3' untranslated under the control of the mouse metallothionein promoter. The construct contains the entire sequence of alpha hCG plus 10 bp 5' untranslated and 220 bp 3' untranslated under the control of the SV40 early promoter. An EcoRI/BamHI fragment of palphat2 (containing alpha hCG and SV40 sequences) was inserted in the BamHI site of pRF398 (ATCC CRL 8401) (containing beta hCG, MT-1 promoter, and BPV sequences) to create pRF398alphat2. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Rinse cell sheet 2 times with fresh 0.25% trypsin, remove trypsin and allow the culture to stand for 5 to 10 minutes at 37C. Add fresh medium, aspirate and dispense into new flasks. |
| Cryopreservation |
Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Name of Depositor |
Genzyme Corp. |
| U.S. Patent Number |
|
| References |
Reddy VB, et al. Heteropolymeric protein. US Patent 4,840,896 dated Jun 20 1989
|
