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The tissue donor suffered from osteogenesis imperfecta (tarda), and was the daughter of Ro Bon (ATCC CRL-1248).

female

The tissue donor suffered from osteogenesis imperfecta (tarda), and was the daughter of Ro Bon (ATCC CRL-1248).

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.