国产精品国产精品一区精品国产自在现偷99精品国产在热2019国产拍偷精品网国产精品视频全国免费观看,国产精品v欧美精品v日韩精品青青精品视频国产久久国产精品久久精品国产亚洲精品国产精品国产欧美精品一区二区三区,国产精品第一页国产亚洲精品国产福利国产精品自拍国产精品视频在线观看亚洲国产精品一区二区久久国产精品国产三级国产专不,国产精品视频大陆精大陆国产国语精品2019精品国产品对白在线285年香蕉精品国产高清自在自线隔壁老王国产在线精品在线观看精品国产福利片,国产三级精品三级在专区精品国产自在现偷国产精品一区二区三区国产日韩精品欧美一区喷水亚洲精品国产精品国自产国产在线精品一区二区不卡

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > H2.8
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
H2.8
H2.8
規(guī)格:
貨期:
編號(hào):B164528
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 H2.8
商品貨號(hào) B164528
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue spleen
Cell Type hybridoma:lymphoblast B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age adult
Strain BALB/c
Applications
ELISA detection zona pellucida glycoprotein 2 ZP2, zona pellucida protein A
Western Blot zona pellucida glycoprotein 2 ZP2, zona pellucida protein A
detection of zona pellucida glycoprotein 2 ZP2, zona pellucida protein A
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
zona pellucida glycoprotein 2
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 10 mM HEPES, 0.15 mg/ml oxaloacetate, 0.05 mg/ml pyruvate, 0.0082 mg/ml bovine insulin and 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
Subculturing Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium at 2 x 105 viable cells/mL. Add medium as the cell density increases. Maintain cultures at cell concentrations between 1 x 105 and 1 x 106 viable cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype IgG1 kappa
Name of Depositor J Dean
Deposited As mouse (B cell); mouse (myeloma)
References

Animal were immunized with bacterially expressed, Hi2+-agarose purified 6-histidine tagged recombinant (amino acids 39-642) human ZP2 (zona pellucida glycoprotein 2)

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
辰溪县| 邻水| 多伦县| 太原市| 和政县| 大同县| 区。| 池州市| 陕西省| 丰都县| 宜宾县| 大埔区| 比如县| 乌恰县| 福贡县| 资溪县| 拉孜县| 衡南县| 锡林郭勒盟| 大同县| 年辖：市辖区| 博兴县| 郓城县| 田阳县| 平南县| 鞍山市| 南木林县| 新蔡县| 三明市| 盐池县| 鄂托克前旗| 清远市| 聂荣县| 醴陵市| 花莲县| 重庆市| 九龙坡区| 西平县| 南涧| 邵阳县| 杭锦后旗|