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HuNS1
HuNS1
規(guī)格:
貨期:
編號:B164806
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 HuNS1
商品貨號 B164806
Organism Homo sapiens, human
Cell Type lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease myeloma
Gender male
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
This line was derived from the GM4672 cell line (Human Genetic Mutant Cell Repository, Coriell Institute for Medical Research) following treatment with ethylmethanesulfonate (EMS).
Clinical Data male
Comments

The cells are HAT sensitive and are negative for Epstein-Barr virus.

According to information provided by the patent depositor (US Patent 4,720,459 dated Jan 19 1988), this cell line did not secrete an immunoglobulin. However, recent testing at ATCC has confirmed the presence of IgG.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine, 4.5g/L glucose, 10 mM HEPES, 1 mM sodium pyruvate, 1.5 g/L sodium bicarbonate, 0.02 mM 2-amino-6-methylmercaptopurine, 85%; fetal bovine serum, 15%
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 10cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Complete Culture Medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 10,12
D7S820: 10
THO1: 9,9.3
TPOX: 8
vWA: 16,19
Name of Depositor Medical College of Wisconsin Research Foundation, Inc.
Deposited As Homo sapiens
U.S. Patent Number
References

Winkelhake JL. Myelomas for producing human/human hybridomas. US Patent 4,720,459 dated Jan 19 1988

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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