| 產(chǎn)品名稱(chēng) |
LCL 8664 |
| 商品貨號(hào) |
B164966 |
| Organism |
Macaca mulatta, monkey, rhesus |
| Cell Type |
B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
2 Cells Contain HERPESVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
lymphoma |
| Age |
5 years |
| Gender |
male |
| Storage Conditions |
liquid nitrogen vapor phase |
| Clinical Data |
male |
| Genes Expressed |
B lymphotropic herpesvirus |
| Cellular Products |
B lymphotropic herpesvirus |
| Comments |
CAUTION: the cells are infected with and produce a B lymphotropic herpesvirus (RhEBV) and may represent a potential biohazard.
Free RhEBV is present in the supernatant, and rhesus B lymphocyte transforming activity is present in the medium.
About 1% of the cells express virus capsid antigen, and all express the virus related nuclear antigen. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 x 105 cells/mL and maintain between 4 x 105 and 2 x 106 cells/mL.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Name of Depositor |
SR Rangan |
| Deposited As |
Macaca mulatta |
| References |
Rangan SR, et al. Epstein-Barr virus-related herpesvirus from a rhesus monkey (Macaca mulatta) with malignant lymphoma. Int. J. Cancer 38: 425-432, 1986. PubMed: 3017870
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