国产精品国产精品一区精品国产自在现偷99精品国产在热2019国产拍偷精品网国产精品视频全国免费观看,国产精品v欧美精品v日韩精品青青精品视频国产久久国产精品久久精品国产亚洲精品国产精品国产欧美精品一区二区三区,国产精品第一页国产亚洲精品国产福利国产精品自拍国产精品视频在线观看亚洲国产精品一区二区久久国产精品国产三级国产专不,国产精品视频大陆精大陆国产国语精品2019精品国产品对白在线285年香蕉精品国产高清自在自线隔壁老王国产在线精品在线观看精品国产福利片,国产三级精品三级在专区精品国产自在现偷国产精品一区二区三区国产日韩精品欧美一区喷水亚洲精品国产精品国自产国产在线精品一区二区不卡

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > pUT11 [URA3 --> TRP1 converter]
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
pUT11 [URA3 --> TRP1 converter]
pUT11 [URA3 --> TRP1 converter]
規(guī)格:
貨期:
編號(hào):B215734
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pUT11 [URA3 --> TRP1 converter]
商品貨號(hào) B215734
Designations pUT11 [URA3 --> TRP1 converter]
Depositors FR Cross
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 6.6999998092651370
DESCRIPTION OF VECTOR:
Intact vector size: 6.700
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Construction: pUC19
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
replicon: pMB1
marker(s): ampR, <-
restriction site: EcoRI, SmaI
gene disruption cassette: ura3::TRP1/kanR
restriction site: SmaI
Vector: pUT11 (plasmid)
Construction: pUC19
Marker(s):TRP1,ampR,kanR
Construct size (kb): 6.699999809265137
Features: gene disruption cassette: ura3::TRP1/kanR
marker(s): ampR
replicon: pMB1
restriction site: EcoRI, SmaI
restriction site: SmaI
Applications
YI-type (integrating) shuttle vector
host modification
marker swap vector URA3 --> TRP1
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--4.6, 2.0; SmaI--3.8, 2.7.
To convert the host phenotype from URA3 to TRP1, transform with the SmaI digested vector and select for Trp+ transformants.
Some combinations of marker swap plasmids and target locus may result in relatively high reversion rates. In most but not all cases the frequencies of successful convertants are greater than 30%.
A marker swap vector designed to change the S. cerevisiae host phenotype by one-step gene disruption of the URA3 gene with the TRP1 and kanR markers.
When swapping markers on an episomal plasmid, appropriate phenotype may result from loss of the plasmid unless a second selectable or scorable marker is used to ensure plasmid maintenance.
Vector was constructed by replacing an internal StuI fragment of URA3 with a SmaI fragment containing the TRP1 and kanR coding sequences. TRP1 and URA3 are in the same orientation.
Media ATCC® Medium 1948: LB medium (ATCC medium 1065) with 50 mcg/ml ampicillin and 20 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Cross FR. 'Marker swap' plasmids: convenient tools for budding yeast molecular genetics. Yeast 13: 647-653, 1997. PubMed: 9200814

Related Products
component of:ATCC 87561
Shipped freeze-dried
Shipping Information Distributed: freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
师宗县| 锡林浩特市| 乐清市| 鄂伦春自治旗| 平武县| 河源市| 民县| 阳江市| 泊头市| 蒲城县| 临潭县| 明水县| 湾仔区| 柘荣县| 曲阜市| 南开区| 峡江县| 木兰县| 荔浦县| 临夏县| 边坝县| 汾阳市| 中超| 建宁县| 临潭县| 无极县| 上犹县| 封丘县| 安平县| 镇宁| 富顺县| 七台河市| 特克斯县| 久治县| 盐源县| 定南县| 响水县| 鄂州市| 中卫市| 佛坪县| 尼勒克县|