国产精品国产精品一区精品国产自在现偷99精品国产在热2019国产拍偷精品网国产精品视频全国免费观看,国产精品v欧美精品v日韩精品青青精品视频国产久久国产精品久久精品国产亚洲精品国产精品国产欧美精品一区二区三区,国产精品第一页国产亚洲精品国产福利国产精品自拍国产精品视频在线观看亚洲国产精品一区二区久久国产精品国产三级国产专不,国产精品视频大陆精大陆国产国语精品2019精品国产品对白在线285年香蕉精品国产高清自在自线隔壁老王国产在线精品在线观看精品国产福利片,国产三级精品三级在专区精品国产自在现偷国产精品一区二区三区国产日韩精品欧美一区喷水亚洲精品国产精品国自产国产在线精品一区二区不卡

produces protein transposase, Tn10

Construct size (kb): 9.199999809265137

DNA: genomic

Insert lengths(kb): 7.199999809265137

Gene product: transposase, Tn10

Target Gene: transposase, Tn10

Restriction digests of the clone give the following sizes (kb): EcoRI--9.2; HindIII--6.6, 2.5; PstI--5.2, 2.95, 0.98; XbaI--6.8, 2.1.

Construct permitting translational fusions of kan to target genes.

Kanamycin resistance is expressed after transpositions in the correct orientation and reading frame into an expressed target gene.

Contains a 2.2 kb mini-Tn10 cassette conferring erythromycin resistance and a promoterless kan gene ('kan) gene, bounded by inverted repeats of the outermost 70 bp of IS10R and embedded (as an EcoRI/HindIII fragment) in hisG and hisD.

Also contains the wild type transposase gene regulated by the Ptac promoter, inducible by IPTG, oriented opposite to 'kan, and separated from 'kan by 4 tandem repeats of the rrnB terminator.

The order of the major features in the insert is: XbaI - HindIII - transposase - Ptac - rrnB terminators - HindIII - 'kan - erm - EcoRI - XbaI.