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Size (kb): 9.31

Vector: pMMB206 (plasmid)

Promoters: Promoter tac

Construction: pMMB190, pUC8

Marker(s): cmlR

Construct size (kb): 9.31

Features: insert detection: lacZ'
marker(s): cmlR
promoter: tac, lacUV5
replicon: oriV, oriT (IncQ)
repressor gene: lacIq
transcription terminator: rrnB

expression vector

shuttle vector

Restriction digests of the clone give the following sizes (kb): EcoRI--9.3; EcoRV--5.8, 3.6; SspI--3.9, 1.8, 1.6, 1.2, 0.9; EcoRI/MluI--8.4, 0.9.

Contains sequences encoding lacIq, a tandem tac lacUV5 promoter region, and lacZalpha to allow direct screening by inactivation of alpha-complementation. Shows a low level of expression without induction.

Sequencing demonstrated that there are 3 stop codons in frame with the truncated bla gene, terminating translation but permitting continued transcription and expression of the cat gene.

Temperature: 37°C