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Construct size (kb): 3.599999904632568

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-7Zf(+)
Intact vector size: 3.000
Type of vector: phagemid
Vector end: EcoRI
Vector end: HindIII
Cloning sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI AsuII ClaII
HindIII BamHI SacI BstXI NsiI
Polylinker sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI AsuII ClaII
HindIII BamHI SacI BstXI NsiI
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
terminator: none
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CAL1L
Gene name: calpactin I, light polypeptide (p11)
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1, cR
Tissue: foreskin keratinocyte
Type of DNA: cDNA
Insert 5' end: EcoRI
Insert 3' end: HindIII
Insert size (kb): 0.55
Cross references: DNA Seq. Acc.: M81457
Nucleotides 1-545 of the insert correspond to
nucleotides 1-545 of M81457.

Insert lengths(kb): 0.550000011920929

Tissue: foreskin keratinocyte

Gene product: calpactin I, light polypeptide (p11) [CAL1L]

Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.9, 0.6; ScaI--2.2, 1.5; NcoI--3.6; XmnI--1.9, 1.5; BamHI--3.5.

The insert contains the following restriction sites (approximate kb from the 5' end): NcoI--0.1; ScaI--0.17; SphI--0.32; XmnI--0.46.

Corresponds to nt 1-545 of the sequence record, including the complete coding region (nt 72-365). The 5' end is linker derived; the 3' end is a naturally occurring site.