国产精品国产精品一区精品国产自在现偷99精品国产在热2019国产拍偷精品网国产精品视频全国免费观看,国产精品v欧美精品v日韩精品青青精品视频国产久久国产精品久久精品国产亚洲精品国产精品国产欧美精品一区二区三区,国产精品第一页国产亚洲精品国产福利国产精品自拍国产精品视频在线观看亚洲国产精品一区二区久久国产精品国产三级国产专不,国产精品视频大陆精大陆国产国语精品2019精品国产品对白在线285年香蕉精品国产高清自在自线隔壁老王国产在线精品在线观看精品国产福利片,国产三级精品三级在专区精品国产自在现偷国产精品一区二区三区国产日韩精品欧美一区喷水亚洲精品国产精品国自产国产在线精品一区二区不卡

Distribution host: Escherichia coli unknown (ATCC 33694)

Size (kb): 7.375

DESCRIPTION OF VECTOR:
Intact vector size: 7.375
Type of vector: phagemid
Cloning sites:
Polylinker sites:
Other unique sites: BamHI
Construction: pRS406
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->
replicon: f1, <-
other: MET15 deleter cassette
replicon: pMB1
marker(s): ampR, <-

Vector: pAD4 (phagemid)

Construction: pRS406

Marker(s):URA3,ampR

Construct size (kb): 7.375

Features: marker(s): URA3
marker(s): ampR
other: MET15 deleter cassette
replicon: f1
replicon: pMB1

YI-type (integrating) shuttle vector

marker deletion vector O-acetylhomoserine sulfhydrylase

Restriction digests of the clone give the following sizes (kb): KpnI--4.2, 3.1; KpnI/SacI--4.2, 3.1, 0.03; ClaI--7.4.

The two step selection process requires a ura3 transformation host (this host can be created using pJL164 (ATCC 87471)). After transformation with the ClaI linearized vector, URA3 integrants are selected on ura- plates.

The designer deletion strain is then recovered by selection on 5-FOA plates (loss of URA3 and MET15 markers by a homologous recombination event).

MET15, MET17 and MET25 are synonymous.

This deleter vector is used to create designer yeast strains with a non-revertable met15 auxotrophic marker deletion.

Temperature: 37°C

component of:ATCC 87472