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Construct size (kb): 47.79999923706055

DNA: cDNA

Insert lengths(kb): 4.5

Tissue: adult kidney

Gene product: epidermal growth factor(urogastrone) [EGF]

Alleles: A1, B1, B2, A2

Restriction digests of the clone give the following sizes (kb): BamHI--18.9, 7.4, 7.2, 5.2, 3.6, 1.4; EcoRI--30.7, 12.1, 4.4; HindIII--24.3, 9.9, 7.6, 4.4, 4.0; BglII--23.1, 12.5, 11.4, 4.7; SmaI--20.9, 8.9, 8.5, 6.9, 5.5.

A 6.4 kb SmaI fragment of this construct (62 bp 5' untranslated, the coding sequence, through 2.0 kb of the right arm of lambdagt10) was inserted into pSAD and transfected into COS-7 cells. Immunoreactive membrane protein was detected.

The insert includes nucleotides 328-4848 of the composite cDNA sequence, encompassing about 115 bp 5' untranslated, the complete coding sequence of 3621 bp, and 791 bp of 3' untranslated sequence.