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Size (kb): 7.0999999046325680

Vector: YIp353 (plasmid)

Promoters: Promoter none

Construction: YEp353 (ATCC 37725), YIp352

Marker(s):URA3,ampR

Construct size (kb): 7.099999904632568

Features: insert detection: lacZ
marker(s): ampR, URA3
promoter: none
replicon: pMB1

YI-type (integrating) shuttle vector

promoter-cloning vector

shuttle vector

The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-2; SmaI-3; BamHI-3; SalI-3; PstI-1; HindIII-2.

Restriction digests of the clone give the following sizes (kb): EcoRI--7.1; BamHI--7.1; SalI--7.1; PstI--7.1; HindIII--7.1.

One of 3 promoter-cloning, YI type shuttle vectors (ATCC 37743 - 37745) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.

The sequence and reading frame of the multiple cloning sequence is: 5' GA ATT CCC GGG GAT CCG TCG ACC TGC AGC CAA GCT TGC GAT CCC 3'. The sequence begins with the first nucleotide of the MCS and ends with the CCC for amino acid 8 of beta-galactosidase.

Temperature: 37.0°C