国产精品国产精品一区精品国产自在现偷99精品国产在热2019国产拍偷精品网国产精品视频全国免费观看,国产精品v欧美精品v日韩精品青青精品视频国产久久国产精品久久精品国产亚洲精品国产精品国产欧美精品一区二区三区,国产精品第一页国产亚洲精品国产福利国产精品自拍国产精品视频在线观看亚洲国产精品一区二区久久国产精品国产三级国产专不,国产精品视频大陆精大陆国产国语精品2019精品国产品对白在线285年香蕉精品国产高清自在自线隔壁老王国产在线精品在线观看精品国产福利片,国产三级精品三级在专区精品国产自在现偷国产精品一区二区三区国产日韩精品欧美一区喷水亚洲精品国产精品国自产国产在线精品一区二区不卡

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > pSTMV5
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
pSTMV5
pSTMV5
規(guī)格:
貨期:
編號:B245010
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pSTMV5
商品貨號 B245010
Designations pSTMV5
GenBank Number

M25782

Species satellite tobacco mosaic tobamovirus (STMV)
Depositors G Kurath
Applications
Linearize with NsiI and transcribe with T7 RNA polymerase to produce a sense strand.
Vector
Construct size (kb): 4.300000190734863
Insert
DNA: cDNA
Insert lengths(kb): 1.059000015258789
Insert Size (kb) 1.059
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): XbaI/SphI--3.4, 1.15; SalI--4.4; BglII--4.4.
The insert contains the following restriction sites (bp from the 5' XbaI site): DraI--321; SalI--602; BglII--791. The primer-derived NsiI site is at nt 1059.
Clones pSTMV5 and pSTMV6 (ATCC 45135, 45136) can be distinguished by RNase protection assays which give distinct patterns due to an A-C difference at nt 751 of the STMV sequence.
The RNA transcribed from this clone is infectious.
Contains a complete STMV genome representing the major genome type within the STMV type strain.
pSTMV5 is identical to the reported STMV type strain sequence except for a G to A difference at nt 494.
Linearize with NsiI and transcribe with T7 RNA polymerase to produce a sense strand.
Constructed using primers for cDNA synthesis that added NsiI and SphI sites at the 3' end and an XbaI site at the 5' end.
Classification Tobamovirus
References

Kurath G, et al. Analysis of genetic heterogeneity within the type strain of satellite tobacco mosaic virus reveals several variants and a strong bias for G to A substitution mutations. Virology 189: 233-244, 1992. PubMed: 1604813

Mirkov TE, et al. Nucleotide sequence and translation of satellite tobacco mosaic virus RNA. Virology 170: 139-146, 1989. PubMed: 2718378

Gael Kurath, personal communication

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
剑阁县| 武威市| 泸水县| 西吉县| 大连市| 陕西省| 商城县| 同江市| 深州市| 航空| 平塘县| 柳林县| 郎溪县| 桂阳县| 正阳县| 东乡族自治县| 株洲市| 张家港市| 垣曲县| 伊宁县| 青铜峡市| 平乡县| 嘉义市| 博湖县| 平武县| 康乐县| 霍州市| 通州区| 绥宁县| 衡阳市| 武功县| 镇雄县| 福建省| 日土县| 韶关市| 石棉县| 太保市| 保康县| 娱乐| 台北县| 晋中市|